SOX6 Downregulation Induces γ-Globin in Human β-Thalassemia Major Erythroid Cells

Figure 1

(a) qPCR analysis showed that downregulation of SOX6 expression in K562 cells induces γ-globin production. (b) Western blot analysis showed that downregulation of SOX6 expression in K562 cells induces γ-globin production. (c) qPCR analysis showed that downregulation of SOX6 expression in primary adult erythroid cells from normal donors induces γ-globin production. (d) qPCR analysis showed that downregulation of SOX6 expression in primary adult erythroid cells from patients with β-thalassemia major induces γ-globin production. (e) Flow cytometry analysis showed that in cells from patients with β-thalassemia major transduced with SOX6 shRNA, there are no obvious changes in CD235a expression as compared to the control group or control vector group. (f) qPCR analysis showed that, in primary adult erythroid cells transduced with SOX6 shRNA, there are no significant differences in CD235a expression as compared to the control group or control vector group. C group: control; CV group: control vector; SH group: shRNA. .

(a)

(b)

(c)

(d)

(e)

(f)