Research Article

SOX6 Downregulation Induces γ-Globin in Human β-Thalassemia Major Erythroid Cells

Table 3

RIPA buffer.

50 mM Tris0.6057 g1.51 g
150 mM NaCl0.8766 g2.19 g
0.5% NaDeoxycholate0.5 g1.25 g
1% NP401 ml2.5 ml
0.1% SDS0.1 g0.25 g
pH to 8dH2O to 100 mldH2O to 250 ml