Role of GntR Family Regulatory Gene SCO1678 in Gluconate Metabolism in Streptomyces coelicolor M145

Figure 7

Gluconate inhibits ACT production in S. coelicolor M145 and triggers production of yellow-pigmented compound, CPK, in S. coelicolor ΔgntR. Spent media in cuvettes are shown (a) where respective strains were grown. The production levels correspond to equal amounts of biomass, as judged by Bradford protein assay. On glucose-containing SMM agar both M145 and ΔgntR produce ACT, and yellow pigment was not obvious (b), while on gluconate-containing Oxoid agar (c) these strains differ in secondary metabolism profile.

(a)

(b)

(c)