Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome

Figure 1

WT1 expression and localization in podocytes from biopsies and urinary sediments of patients with NS. (a) Immunofluorescence of renal biopsies (magnification, ×40) of patients with nephrotic syndrome (NS). (b) Immunofluorescence of podocytes in urine sediments: nephrin detection was used as a second marker of podocytes (magnification, ×40). (c) Colocalization of DAPI and Wilms tumor 1 (WT1) in nucleus/cytoplasm of podocytes in kidney biopsies. (d) Colocalization of DAPI and WT1 in nucleus/cytoplasm of podocytes in urinary sediments. The colocalization was determined by Pearson correlation coefficient (PCC). , , and .

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