Treatment with Sildenafil and Donepezil Improves Angiogenesis in Experimentally Induced Critical Limb Ischemia

Figure 1

Techniques used for the histopathological study. Gastrocnemius muscle cross-sections: H&E staining for the evaluation of the number of nuclei/field; immunohistochemical staining for endothelial cells (CD31), dividing cells (Ki-67) and vascular endothelial growth factor (VEGFR-3).