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BioMed Research International
Volume 2017, Article ID 9538351, 11 pages
https://doi.org/10.1155/2017/9538351
Research Article

Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes

1Department of General Biology, Federal University of Viçosa, 35570-000 Viçosa, MG, Brazil
2Institute of Biomedical Sciences, Department of Structural Biology, Federal University of Alfenas, 37130-001 Alfenas, MG, Brazil
3Department of Biochemistry and Molecular Biology, Federal University of Viçosa, 35570-000 Viçosa, MG, Brazil
4Center of Biomolecules Analysis (NuBioMol), Federal University of Viçosa, 35570-000 Viçosa, MG, Brazil
5Department of Animal Biology, Federal University of Viçosa, 35570-000 Viçosa, MG, Brazil

Correspondence should be addressed to Reggiani V. Gonçalves; rb.moc.oohay@alelivsygger

Received 15 April 2017; Revised 28 June 2017; Accepted 13 July 2017; Published 13 September 2017

Academic Editor: Antoni Camins

Copyright © 2017 Mariáurea M. Sarandy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The effect of topical application of ointment based on Strychnos pseudoquina hydroethanolic extract in the cutaneous wounds healing in diabetic rats was evaluated. Samples of S. pseudoquina were submitted to phytochemical prospection and in vitro antioxidant assay. Thirty Wistar rats were divided into 5 groups: Sal-wounds treated with 0.9% saline solution; VH-wounds treated with 0.6 g of lanolin cream (vehicle); SS-wounds treated with silver sulfadiazine cream (10 mg/g); ES5- and ES10-wounds treated with an ointment of S. pseudoquina extract, 5% and 10%, respectively. Fragments of wounds were removed for histological and biochemical analysis every 7 days during 21 days. ES showed equivalent levels per gram of extract of total phenols and flavonoids equal to 122.04 mg for TAE and 0.60 mg for RE. The chlorogenic acid was one of the major constituents. S. pseudoquina extract presented high antioxidant potential in vitro. ES5 and ES10 showed higher wound healing rate and higher amount of cells, blood vessels, and type III and I collagen. The oxidative stress markers were lower in the ES5 and ES10 groups, while the antioxidants enzymes levels were higher. Ointment based on S. pseudoquina extract promotes a fast and efficient cutaneous repair in diabetic rats.