Inhibition of Pseudomonas aeruginosa Biofilm Formation by Traditional Chinese Medicinal Herb Herba patriniae

Table 2

Reporter genes and primer sequences used.

PA; number	Gene	Function	Primer	Sequence
PA0762	algU	RNA polymerase sigma factor	pKD-algU-S	GCACTCGAGAGGATGCCTGAAGACCTC
			pKD-algU-A	GTAGGATCCGATGGCGATCCGATACAG
PA2243	pslM	Succinate dehydrogenase; fumarate reductase flavoprotein	pKD-pslM-S	ATCCTCGAGCGGTGCGCAAGAAGACC
			pKD-pslM-A	GTTGGATCCCGTAACGCTCGCCCAGTT
PA3064	pelA	Glycoside hydrolase; deacetylase	pKD-pelA-S	CGTCTCGAGCTTTCCACTTTGCCACAG
			pKD-pelA-A	TACGGATCCTACCAGAACGCCACGCT
PA3551	algA	Phosphomannose isomerase; guanosine 5′-diphospho-D-mannose pyrophosphorylase	pKD-algA-S	TGACTCGAGTGAAGGCGGACTGAGAC
			pKD-algA-A	TATGGATCCGCGACCAGTTTCATC
PA2663	ppyR	psl and pyoverdine operon regulator	pKD-ppyR-S	GCACTCGAGCACTTCTTCTGCTACAGC
			pKD-ppyR-A	GTAGGATCCAGCACTTGCACAGCAGAC
PA1423	bdlA	Biofilm dispersion locus A	pKD-bdlA-S	GCACTCGAGCGTCATATTTCCGACGAA
			pKD-bdlA-A	TCAGGATCCGTAGTCTTCCGATTGCG