Tempol, a Superoxide Dismutase Mimetic Agent, Inhibits Superoxide Anion-Induced Inflammatory Pain in Mice

Figure 3

Tempol inhibits KO₂-induced COX-2 and preproET-1 mRNA expressions in the paw skin and spinal cord. Mice were treated with tempol (100 mg/kg) or vehicle 40 min before KO₂ intraplantar injection. Paw skin and spinal cord samples were collected 3 h after KO₂-stimulus and COX-2 ((a) and (b)) and preproET-1 ((c) and (d)) mRNA expressions were evaluated by RT-qPCR. Results were normalized using GAPDH as gene control and expressed as means ± SEM ( per group per experiment, representative of two separate experiments) [ versus saline; versus KO₂ + vehicle (one-way ANOVA followed by Tukey’s -test)].

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