Tempol, a Superoxide Dismutase Mimetic Agent, Inhibits Superoxide Anion-Induced Inflammatory Pain in Mice

Figure 4

Tempol inhibits KO₂-induced depletion of antioxidant capacity in the paw skin and spinal cord. Mice were treated with tempol (100 mg/kg) or vehicle 40 min before KO₂ intraplantar injection. Paw skin and spinal cord samples were collected 3 h after KO₂-stimulus and the oxidant capacity was evaluated by FRAP ((a) and (b)), ABTS ((c) and (d)), and GSH ((e) and (f)) assays. Results are expressed as means ± SEM ( per group per experiment, representative of two separate experiments) [ versus saline; versus KO₂ + vehicle (one-way ANOVA followed by Tukey’s -test)].

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