Cell Signaling Pathway in 12-O-Tetradecanoylphorbol-13-acetate-Induced LCN2 Gene Transcription in Esophageal Squamous Cell Carcinoma

Figure 5

The analyses of MAPK (mitogen-activated protein kinase) signal pathway proteins in TPA-treated EC109 cells. Total protein from EC109 cells treated with TPA for 0 h, 3 h, 6 h, 12 h, and 24 h was obtained; then ERK1/2 (extracellular regulated protein kinases 1 and 2), p-ERK1/2 (phospho-ERK1/2), and p-JNK (phospho- c-jun N-terminal kinase) were detected by western blotting, respectively. β-Actin was shown as a loading control.