RNA-Binding Protein Dnd1 Promotes Breast Cancer Apoptosis by Stabilizing the Bim mRNA in a miR-221 Binding Site

Figure 1

Dnd1 expression is correlated with Bim expression in breast cancer tissues. (a) After annotating the gene microarrays according to the TCGA microarray subtypes, we identified the expression levels of Dnd1 which were lower in recurrence groups than in no-recurrence groups. (b) The expression of Dnd1 and Bim in breast cancer tissues includes positive correlation (). (c) Correlation between the levels of Dnd1 and the survival of breast cancer patients. When the expression of Dnd1 was considered, patients in the Dnd1 low-expression group had a significantly shorter survival time (). (d and e) The Dnd1 expression levels were determined in normal and breast cancer cells by qRT-PCR and western blot. The expression levels of Dnd1 are higher in normal mammary gland epithelial cells than in breast cancer cells. (f) qRT-PCR analysis of Dnd1 mRNA with whole RNA extracted from mouse breast tumor cell lines with different metastatic potential. (g) Dnd1 mRNA level is decreased in human breast tumor samples. Twenty-one pairs of breast tumors with adjacent mammary gland epithelial tissues were analyzed by qRT-PCR. Values are mean ± SD. (, one-way ANOVA).

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