Detecting the Candidate Gender Determinants by Bioinformatic Prediction of miRNAs and Their Targets from Transcriptome Sequences of the Male and Female Flowers in Salix suchowensis
Table 1
Potential miRNAs identified in female flower buds of S. suchowensis.
miRNA
miRNA sequence (5′-3′)
LM (nt)
Arm
Pre-miR location
LP (nt)
ssu-miR162a
UCGAUAAACCUCUGCAUCCAG
21
3′
seq459792:217..303:+
86
ssu-miR162b
UCGAUAAACCUCUGCAUCCAG
21
3′
seq351845:128..215:−
87
ssu-miR162c
UCGAUAAACCUCUGCAUCCAG
21
3′
seq365695:26..113:+
87
ssu-miR166
GGAAUGUUGUCUGGCUCGAGG
21
5′
seq576601:133..255:+
122
ssu-miR169
CAGCCAAGGAUGACUUGCCGG
21
3′
seq292249:428..483:-
55
seq292249:488..543:−
ssu-miR390
CGCUAUCCAUCCUGAGUUUCA
21
3′
seq242088:345..447:−
102
ssu-miR172a
AGAAUCCUGAUGAUGCUGCAG
21
5′
seq288542:116..254:+
138
seq263722:286..424:+
seq600570:208..346:−
ssu-miR472a
UUUUCCCAACUCCACCCAUCCC
22
3′
seq62383:374..435:−
61
ssu-miR472b
UUUUCCCAACUCCACCCAUCCC
22
3′
seq355906:377..445:−
68
ssu-miR1448
CUUUCCAACGCCUCCCAUAC
20
3′
seq140125:105..174:+
69
ssu-miR6423
CCGCUGUCGCCACUAUCUUCCU
22
5′
seq431318:268..328:+
60
seq593886:92..152:+
LM: length of miRNA; Arm: location of mature miRNAs on secondary stem-loop structures of pre-miRNA sequences; Pre-miR location: miRNA precursor’s location in ESTs of willow female flower buds; LP: length of pre-miRNA.
