Detecting the Candidate Gender Determinants by Bioinformatic Prediction of miRNAs and Their Targets from Transcriptome Sequences of the Male and Female Flowers in Salix suchowensis

Table 2

Potential miRNAs identified in male flower buds of S. suchowensis.

miRNA	miRNA sequence (5′-3′)	LM (nt)	Arm	Pre-miR location	LP (nt)
ssu-miR156a	UGACAGAAGAUAGAGAGCAC	20	5′	seq517259:84..165:+	81
ssu-miR156b	UGACAGAAGAUAGAGAGCAC	20	5′	seq325710:29..80:−	51
ssu-miR160	UGCCUGGCUCCCUGUAUGCC	20	5′	seq145824:369..444:−	75
ssu-miR167a	AGAUCAUGUGGCAGUUUCACC	21	3′	seq137705:68..140:+	72
				seq383115:96..168:+
ssu-miR167b	AGAUCAUGUGGCAGUUUCACC	21	3′	seq450474:97..169:+	72
ssu-miR167c	AGAUCAUGUGGCAGUUUCACC	21	3′	seq460555:421..495:−	74
				seq460555:481..555:−
ssu-miR162a	UCGAUAAACCUCUGCAUCCAG	21	3′	seq430376:217..303:+	86
ssu-miR162d	UGGAGGCAGCGGUUCAUCGAUC	22	5′	seq344645:410..500:+	90
ssu-miR169a	CAGCCAAGGAUGACUUGCCGG	21	5′	seq159785:89..210:−	121
ssu-miR169b	CAGCCAAGGAUGACUUGCCGG	21	5′	seq167083:107..214:+	107
				seq166207:31..138:+
ssu-miR172b	GCAGCACCAUCAAGAUUCACA	21	5′	seq555414:28..136:+	108
ssu-miR172c	GCAGCACCAUCAAGAUUCACA	21	5′	seq562308:14..122:−	108
ssu-miR396a	UUCCACAGCUUUCUUGAACUG	21	3′	seq114772:89..196:+	107
ssu-miR396b	UUCCACAGCUUUCUUGAACUG	21	3′	seq307347:218..305:−	87
ssu-miR472a	UUUUCCCAACUCCACCCAUCCC	22	3′	seq467610:63..125:+	62
ssu-miR472c	UCUUGCCUACUCCUCCCAUUCC	22	3′	seq106361:38..109:+	71
ssu-miR1448a	CUUUCCAACGCCUCCCAUAC	20	3′	seq106361:219..287:+	68
ssu-miR1448b	CUUUCCAACGCCUCCCAUAC	20	3′	seq83628:392..461:−	69
				seq83628:422..491:−
ssu-miR7841	GGGGGUUGCUGUCAAGCAUAA	21	3′	seq306990:54..154:−	100
ssu-miR6423a	CCGCUGUCGCCACUAUCUUCCU	22	5′	seq268392:35..125:+	90
ssu-miR6423b	CCGCUGUCGCCACUAUCUUCCU	22	5′	seq228745:13..103:+	90
ssu-miR6423c	CCGCUGUCGCCACUAUCUUCCU	22	5′	seq199601:0..96:+	96
ssu-miR6423d	CCGCUGUCGCCACUAUCUUCCU	22	5′	seq100248:45..105:−	60
				seq148917:45..105:−

LM: length of miRNA; Arm: location of mature miRNAs on secondary stem-loop structures of pre-miRNA sequences; Pre-miR location: miRNA precursor’s location in ESTs of willow male flower buds; LP: length of pre-miRNA.