Role of MicroRNA-103a Targeting ADAM10 in Abdominal Aortic Aneurysm

Figure 2

ADAM10 was involved in the formation of murine AAA. (a) Comparison of AAA sizes with/without ADAM10 inhibitor GI254023X by naked eye; (b) the representative ultrasonic images of AAA diameter with/without GI254023X on day 14 of Ang II infusion; (c) comparison of AAA diameters with/without GI254023X by ultrasonic measurement day 14 (AngII, ; control, ; AngII + GI254023X ; p, AngII/control, 0.0000; AngII + GI254023X/control, 0.0010; AngII/AngII + GI254023X, 0.0000), Ang II group, , control group, , and AngII + GI254023X group, ; day 21 (AngII, ; control, ; AngII + GI254023X, ; p, AngII/control, 0.0000; AngII + GI254023X/control, 0.0000; AngII/AngII + GI254023X, 0.0000), Ang II group, , control group, , and AngII + GI254023X group, ; (d) comparison of blood pressure and heart rate with/without GI254023X. SBP is systolic blood pressure (AngII, ; control, ; AngII + GI254023X, ; p, AngII/control, 0.0030; AngII + GI254023X/control, 0.4585; AngII/AngII + GI254023X, 0.0111), AVG HR is average heart rate (AngII, ; control, ; AngII + GI254023X, ; p, AngII/control, 0.1917; AngII + GI254023X/control, 0.8999; AngII/AngII + GI254023X, 0.1023), AngII group, , control group, , and AngII + GI254023X group, ; (e) examples of IHC staining of Mac-2 in AAA macrophages with/without GI254023X (scale bar 10 μm) (AngII, ; AngII + GI254023X, ; p, 0.0151), AngII group, , and AngII + GI254023X group, ; (f) comparison of ADAM10 protein expressions with/without GI254023X by Western blot analysis (AngII, ; AngII + GI254023X, ; p, 0.002), AngII group, , and AngII + GI254023X group, ; (g) comparison of TNF-α protein expressions with/without GI254023X by Western blot analysis (AngII, ; AngII + GI254023X, ; p, 0.06), AngII group, , and AngII + GI254023X group, ; (h) comparison of CX3CL1 protein expressions with/without GI254023X by Western blot analysis (AngII, ; AngII + GI254023X, ; p, 0.02), Ang II group, , and AngII + GI254023X group, ; (i) comparison of IL-6R protein expressions with/without GI254023X by Western blot analysis (AngII, ; AngII + GI254023X, ; p, 0.04), AngII group, , and AngII + GI254023X group, ; (j) comparison of VE-cadherin protein expressions with/without GI254023X by Western blot analysis (AngII, ; AngII + GI254023X, ; p, 0.04). GAPDH is the reference protein; , , AngII group, , and AngII + GI254023X group, .

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