Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells

Table 1

Oligonucleotide sequences used for RT-qPCR analysis.

Gene	Gene accession number	Primer sequence (5′-3′)	Product size (bp)
CYP19	NM_214429.1	AAAGACGCAGGATTTTCACA; TCTTTTGTCAGTTCACCACGT	97
FSHR	NM_214386.2	GAATTGAAAAGGCCAACAAC; CTTTCAAAACTTAGTCCCACG	214
LHR	NM_214449.1	AAGCACAGCAAGGAGACCAA; AAGAGGACAGTCACGTTTCC	231
Cx36	XM_001924757.4	AGCAGCACTCCACTATGATCGG; TGTTGCACACAAACATGGTCT	123
Cx37	NM_001244224.1	GTGTGTCCGCACCGCCACCGATGA; ACAGGACCGTCAGCCAGA	155
Cx40	XM_003130802.2	ATCGCTTTCACCTGCAAGTCC; CGCCATCCTCAGCAGAACCAT	220
Cx43	NM_001244212.1	CAGCACTTTTCTTTCATTAGGGGC; CTAAGGACTCCAGTCACC	194
ACTB	XM_003124280.3	CCCTTGCCGCTCCGCCTTC; GCAGCAATATCGGTCATCCAT	69
PBGD	NM_001097412.1	GAGAGTGCCCCTATGATGCT; ATGATGGCACTGAACTCCT	214