Skin Tissue Engineering: Application of Adipose-Derived Stem Cells

Figure 1

Development of a three-dimensional prevascularized dermo-epidermal skin substitute. Primary cells including epidermal keratinocytes, melanocytes, dermal fibroblasts, and endothelial cells can be isolated from a single skin biopsy. Dermal fibroblasts and endothelial cells are embedded into a collagen type 1 hydrogel to create a prevascularized dermal compartment. After they remodeled the collagen matrix, keratinocytes and melanocytes are then seeded onto it to create a pigmented epidermal layer.