Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal Stem/Progenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity

<div>P2 cell cluster morphologies at different densities. (a) P2-LD NPMSC clusters showing a turbulence arrangement with an elongated spindle shape and high homogeneity. (b) P2-MD NPMSC clusters showing a short spindle shape with an inconspicuous turbulence arrangement. (c) P2-HD NPMSC clusters showing flattened and enlarged cells with low refractivity and higher heterogeneous shape. All images were taken at a magnification of 100x.</div>

BioMed Research International

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Figure 4: Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal Stem/Progenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity 

Figure 4 | Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal Stem/Progenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity 