Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal Stem/Progenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity

Figure 7

Determination of proliferative capacity of NPMSCs at different densities using the CCK-8 assay. While the OD value of LD group was slightly higher than that of the MD and HD groups at the logarithmic phase, no significant difference was noted between the groups. All samples examined in triplicate.