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BioMed Research International
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BioMed Research International
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2017
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Article
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Tab 1
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Research Article
Use of Limiting Dilution Method for Isolation of Nucleus Pulposus Mesenchymal Stem/Progenitor Cells and Effects of Plating Density on Biological Characteristics and Plasticity
Table 1
Primer sequences for qRT-PCR.
Gene name
Forward sequence
Reverse sequence
β
-Actin
AgATCCTgACCgAgCgTggC
CCAgggAggAAgAggATgCg
Nanog
TCTggggACCTACCTCTT
CTTggTgAggACCTTgTTC
Sox2
gCACCgCTACgACgTCAg
gCCTCggACTTgACCACA
Oct4
ggACACCTggCTTCAgACTT
TCCACAgAACTCgTATgCTg