MicroRNA-29b Contributes to Collagens Imbalance in Human Osteoarthritic and Dedifferentiated Articular Chondrocytes

Table 1

Microarray screening of miRNAs in human IL-1β–stimulated chondrocytes. Total RNAs were isolated from “non-OA” human chondrocytes in primary culture challenged or not with 10 ng/ml of human recombinant interleukin-1β (hrIL1-β) for 48 h. Microarray analysis of miRNAs was performed with the miRCURY LNA Array 11.0 (Exiqon). Data are expressed as the ratio of chondrocyte miRNA levels measured in IL1-β over basal conditions. A/miRs being upregulated by more than 1.2-fold; B/miRs being downregulated by a factor lower than 0.8-fold. Values for miRNA levels are the median of 4 raw signal intensities.

(a)

miRNA	IL-1β stimulated chondrocytes (fold-change)	Unstimulated chondrocytes (fold-change)	Ratio; IL-1β stimulated/unstimulated
miR-146a	1.64	0.52	3.16
miR-155	1.36	0.57	2.38
miR-147b	1.22	0.67	1.83
miR-183	1.59	0.91	1.73
miR-146b-5p	1,32	0.77	1.71
miR-let7i	1.14	0.79	1.44
miR-29b	1.39	1.00	1.39
miR-886-5p	0.73	0.53	1.37
miR-568	1.14	0.87	1.32
miR-495	1.00	0.81	1.24

(b)

miRNA	IL-1β stimulated chondrocytes (fold-change)	Unstimulated chondrocytes (fold-change)	Ratio of; IL-1β stimulated/unstimulated
miR-23a	0.69	1.19	0.58
miR-140-5p	1.07	1.42	0.75
miR-455-3p	1.12	1.48	0.75
miR-140-3p	1.11	1.47	0.76
miR-199a-5p	0.94	1.24	0.76
miR-455-5p	1.05	1.36	0.77
miR-602	0.97	1.24	0.79
miR-1184	0.98	1.25	0.79
miR-199a-3p	1.14	1.42	0.80