The Phosphatase Inhibitor Calyculin-A Impairs Clot Retraction, Platelet Activation, and Thrombin Generation

Figure 5

The effects of TRAP, thiomersal activation, and CLA preincubation on phosphatidylserine-expression (PS). The positivity of platelet PS-expression according to the Annexin-V labeling is in the upper right quadrants of the dot plots. The PS-expression of nonactivated sample (NA) was very low. When platelets were preincubated with CLA the PS-expression further decreased. Representative dot plots show that TRAP activation resulted in elevated PS-expression, which was completely blocked by preincubation with CLA. Thiomersal activated almost all of the platelets and this activation could not be inhibited by CLA preincubation.