Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications

Table 2

Summary of ECM type treated using main decellularization strategies.

Agents and techniques	Cell sheet/tissue/organ
SDS	Fibroblast cell sheet [55], rat forearm [58], porcine cornea [30], porcine myocardium [59], porcine heart valve [47], porcine small intestine (SIS) [45], porcine kidney [34], human vein [60], rat, porcine, and human lungs [40, 46], human heart [61]
Triton X-100	Bovine pericardium [48], porcine kidney [34]
SD/DNase	Porcine blood vessel [63], bovine blood vessel [49], rat trachea [64], porcine trachea [50], rat small intestine [51], porcine SIS [45]
CHAPS	Rat lung [40, 41], human and porcine lung [40, 46]
Peracetic acid	Porcine SIS [45], porcine urinary bladder [52]
EDTA/trypsin	Porcine pulmonary valve [47, 53]
Freeze-thaw	Fibroblast cell sheet [55], canine lumbar spinal segment [56]
High hydrostatic pressure	Porcine cornea [28], porcine blood vessel [23]