Evidence for Tissue Toxicity in BALB/c Exposed to a Long-Term Treatment with Oxiranes Compared to Meglumine Antimoniate

Figure 7

Histopathological analysis of mice brain and cerebellum. A regular architecture of the cerebral and cerebellar cortex of nontreated BALB/c mice is shown (A, G). Treated mice with epoxy-α-lapachone (B, C) and epoxymethoxy-lawsone (D, E) showed thickening of pia mater and reactive gliosis. No alterations were observed. Meglumine antimoniate in the cortex (F). Cerebellum of mice treated with oxiranes showed degeneration in Purkinje neurons (H, I). Alterations detected in brain and cerebellum of animals treated with oxiranes indicated a blood-brain barrier disruption. Brain tissues were stained with hematoxylin and eosin. Tissue images are representative of 10-fragment analysis by animals (), for each treatment group. Cortex region (cr), degenerate Purkinje neuron (dp), granular layer (gl), infiltrates (inf), molecular layer (mL), pia mater (pm), Purkinje neuron (pn), reactive gliose (rg), vein (v), and white matter (wm).