Pterostilbene Induces Cell Apoptosis and Cell Cycle Arrest in T-Cell Leukemia/Lymphoma by Suppressing the ERK1/2 Pathway

Figure 3

Pterostilbene and SCH772984 induce caspase-dependent apoptosis in T-cell leukemia/lymphoma cells. (a) Jurkat cells (3 × 10⁵ cells/mL) were treated with pterostilbene (0, 20, 40, and 80 μM) and incubated for 24 h or 48 h. Data were shown as mean ± SD, . , compared to the control group. , compared to the 24 h group. (b) Hut-78 cells (3 × 10⁵ cells/mL) were treated with pterostilbene (0, 20, 40, and 80 μM) and incubated for 24 h or 48 h. Pterostilbene induced apoptosis of Jurkat and Hut-78 cells in a dose- and time-dependent manner. (c) Jurkat and Hut-78 cells were treated with SCH772984 10 μM for 48 h. Data were shown as mean ± SD, . , compared to the control group. (d) PBMCs and CD34+ cells from peripheral stem cells were treated with pterostilbene (0, 20, 40, and 80 μM), and pterostilbene was of toxicity to normal cells. Data were shown as mean ± SD, . , compared to the control group. (e) Protein levels treated with pterostilbene (0, 20, and 40 μM) of cleaved caspase-3, cleaved caspase-8, caspase-9, and PARP for 48 h were detected by western blot.

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