Dihydromyricetin Attenuates TNF-α-Induced Endothelial Dysfunction through miR-21-Mediated DDAH1/ADMA/NO Signal Pathway

Figure 2

DMY attenuates TNF-α-impaired tube formation and migration in a miR-21 and NO-dependent manner in HUVECs. (a) Representative images show the effect of DMY, miR-21 agomir and antagomir, and L-NAME on tube formation induced by TNF-α (magnification 20x; scale bar, 150 μm); (b) representative images show the effect of DMY, miR-21 agomir and antagomir, and L-NAME on migrated cells on transwell membranes induced by TNF-α (magnification 20x; scale bar, 150 μm). (c) The number of tubes was counted in (a). (d) The number of migrated cells was counted in (b). (e) The transfected efficiency of miR-21 agomir and antagomir on miR-21 expression in HUVECs treated with TNF-α. Data was expressed as mean ± SD, , versus control, versus TNF-α, versus TNF-α + DMY (25 μM), and versus TNF-α + miR-21 antagomir. versus agomir NC, versus antagomir NC, versus TNF-α + miR-21 agomir NC, and versus TNF-α + miR-21 antagomir NC in (e).

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