Mice Selected for Acute Inflammation Present Altered Immune Response during Pristane-Induced Arthritis Progression

Figure 1

Flow cytometry gating strategy for lymphoid cells analysis. Live cells were gated on total cells plot identified by forward scatter (FSC) and side scatter (SSC) properties (a). Cell doublets were excluded by the analysis of the FSC height values over the FSC area values (b). Negative cells to F4-80 and Gr1 markers were selected (c) and then T and B lymphocytes (CD3 and CD19 positive cells, respectively) were analysed (d). In the next step, CD4 and CD8 expression was evaluated inside CD3 positive T cells gate (e).