The Removal of HBV in Plasma by Extracorporeal Immunoadsorption from Plasma: A Potential Therapy of Hepatitis B Patients

Figure 1

Sepharose 6 FF was activated by CNBr through their hydroxyl groups (-OH). (a) Schematic representation of anti-HBsAg functionalized sepharose 6 FF. (b) Time-point optimization of the incubation conditions. 30 min as the optimal time for maximal absorption. Shown is representative of at least 3 individual experiments. (c) Scanning electron micrograph (SEM) images showing surface morphology. (A) (2000X) and (D) (8000X) showed bare CNBr-functionalized sepharose 6 FF beads; (B) (2000X) and (E) (8000X) showed anti-HBsAg functionalized sepharose 6 FF beads; and (C) (2000X) and (F) (8000X) showed anti-HBsAg functionalized sepharose 6 FF beads adsorbed with plasma.

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