Apoptotic and Anti-Inflammatory Effects of Eupatorium japonicum Thunb. in Rheumatoid Arthritis Fibroblast-Like Synoviocytes

Figure 1

Screening of substances with apoptotic effects in the natural product library. (a) MH7A cells were treated with the indicated concentrations of natural product extracts for 24 h. The cell viability was determined by using MTT assay. Compounds GLF, MSK, BCB, GGL, CML, and EJT, corresponding to Glehnia littoralis Fr. Schm., Magnolia sieboldii K. Koch., Boswellia carterii Birdw., Gomphrena globose L., Chelidonium majus L., and Eupatorium japonicum Thunb. (b) MH7A cells were treated with the indicated concentration of EJT extract for 12 h and then stained with Annexin V 488 (green) and Hoechst 33342 (blue) at 25°C (room temperature) for 30 min. The staining intensity of Annexin V in the membrane was observed by confocal laser scanning microscope (magnification 400×). (c) Annexin V intensity in the cell membrane region was analyzed by using the Operetta high-content imaging system.

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