Apoptotic and Anti-Inflammatory Effects of Eupatorium japonicum Thunb. in Rheumatoid Arthritis Fibroblast-Like Synoviocytes

Figure 2

EJT extract induced ROS-dependent apoptosis in MH7A cells. (a) MH7A cells were treated with 37.5 μg/ml EJT extract for 6 h and then treated with 20 μM DCF-DA for 30 min. The intensity of intracellular ROS was detected by using flow cytometry. (b) MH7A cells were pretreated with 20 μM DCF-DA for 2 h and then treated with 37.5 μg/ml EJT extract for 6 h. The intensity of intracellular ROS was observed by using a fluorescence microscope (magnification 400×). (c) MH7A cells were pretreated with 5 mM NAC for 2 h and then treated with 37.5 μg/ml EJT extract for 24 h. Cell viability was determined by using the MTT assay. Significant differences were indicated as . (d) The cell lysates were subjected to SDS-PAGE and the separated samples were analyzed by immunoblotting for antibodies specific to PARP, caspase 7, and GAPDH.

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