Effects of Digeda-4 Decoction on the CYP450 Activities in Rats Using a Cocktail Method by HPLC

Table 2

Precision and extraction efficiency of five probe drugs in rat plasma (n=5).

Compound	Concentration; (μg/mL)	Intraday precision		Interday precision		Extraction efficiency	RSD%
			RSD%		RSD%
Theophylline	5	5.23±0.40	7.65	5.19±0.50	9.63	103.14±8.91	8.64
	25	23.31±1.35	5.79	22.74±1.79	7.87	92.08±6.09	6.61
	100	102.28±4.65	4.55	103.21±4.64	4.50	102.72±4.62	4.50
Tolbutamide	5	4.79±0.30	6.26	4.77±0.40	8.39	96.10±6.63	6.90
	25	24.73±1.31	5.30	26.10±1.49	5.70	101.65±5.79	5.70
	100	101.37±5.27	5.20	98.52 ±6.60	6.70	99.93±5.90	5.90
Chlorzoxazone	5	5.21±0.40	7.67	4.87±0.30	6.16	100.62±6.84	6.80
	25	25.23±1.08	4.28	24.81±1.19	4.80	99.73±4.49	4.50
	100	97.84±3.42	3.50	97.76±4.10	4.19	97.84±3.83	3.91
Omeprazole	0.5	0.52±0.03	5.77	0.47±0.02	4.26	95.33±5.15	5.40
	5	4.48±0.29	6.47	4.31±0.26	6.03	86.59±5.28	6.10
	20	18.79±1.33	7.07	18.86±1.28	6.79	95.13±6.35	6.67
Midazolam	0.5	0.48±0.04	8.33	0.49 ±0.04	8.16	94.53±8.12	8.59
	5	5.58±0.23	4.12	5.63±0. 17	3.02	111.41±2.95	2.65
	20	21.87±1.56	7.13	22.30±1.72	7.71	111.39±9.82	8.82