| strain
(breeder) | age | R | cisplatin
dose | TP | BUN
mg/dl | Cr
mg/dl | end | M | histology and other comments | Ref. | | sex | | N |
| Han-Wistar
(Harlan) | 7wks
female
N=10 | ip | 2.5 mg/kg | D5
D8
D22 | Ns
Ns
ns | Ns
Ns
ns | D22 | 0% | Various doses (0.1; 1; 2.5mg/kg): the effects appeared in a temporal and dose-dependent manner; Histology: D5: mild ATN, D8: minimal ATN, D22: regeneration; majority of alteration seen in the S3 segment of proximal tubules, rarely in distal tubules and collecting ducts | [122, 123] |
| Han-Wistar
(Crl) | 7wks
Male
N=10 | ip | 3 mg/kg | D2
D3
D5 | Ns
Ns
↑3.5x | Ns
Ns
↑2.5x | D5 | 0% | Histology: D2,D3,D5: moderate ATN,
Urine: ↑leukocytes, ↑ total protein (5.6x) | [104] |
| Sprague-Dawley
(Crl) | 6wks
Male
N=10 | ip | 3 mg/kg | D2
D3
D5 | Ns
↑1.37x
↑2.2x | Ns
↑1.25x
↑2.4x | D5 | 0% | Histology: D3-D5: minimal to moderate degeneration/necrosis of the S3 segment of proximal tubules in less than half of rats
Urine: ↑leukocytes, ↑ total protein (4.6x) | [104] |
| Wistar
WKY
(Spain) | 7wks
female
N=4 | ip | 5 mg/kg | D5 | ↑2x | ↑3.5x | D5 | 0% | Histology: tubule swelling, loss of brush border membrane, epithelial vacuolization, hyaline casts and cell debris detachment in proximal tubular cells; ↓GFR 3.3x
Urine:↑volume (1.9x), proteins (1.9x) | [36, 124] |
| Wistar
( Spain) | 7wks
male
N=8 | ip | 5mg/kg | D5 | ↑2.4x | ↑3.3x | D5 | 0% | Histology: tubular necrosis, swelling and tubular
dilatation, extensive epithelial vacuolization,
hyaline casts in tubules;
↓BW (8.5%); ↓GFR (4.1x)
Urine: ↑Na+, ↑volume (2x), ↑proteins (1.7x), ↑urine hydrogen peroxide production, ↓antioxidant capacity in urine | [54] |
| F344
(NIH) | Male
N=7-45 | ip | 6 mg/kg | D1
D2
D3
D4
D5
D7 | Ns
↑1.5x
↑4x
↑3.5x
↑5.3x
↑3.2x | Ns
Ns
↑3.7x
↑4.7x
↑4.7x
↑2.6x | D7 | 0% | ↓5 g of BW per day;
Histology: D3: focal area of mild ATN in proximal tubules: loss of the brush border, necrotic cells sloughing into the tubular lumen, condensed cytoplasm of many cells, increased number of cytoplasmic vesicles in injured cells;
D5: widespread moderate ATN in proximal as and distal tubules: desquamation of necrotic cells, patches of denuded basement membrane, necrotic debris filling the tubular lumens and hyaline casts. Some cells show similar changes as those at D3, lower in height, brush border loss, increased vesicles and swelling;
D7: first signs of regeneration, necrotic cells and necrotic debris are still present | [10, 32] |
| Sprague Dawley
(Turkey) | 8wk,
Male
N=6 | ip | 7 mg/kg | D5 | 107±5.1 | 1.4±0.16 | D5 | 0% | Histology: necrosis in proximal tubules, karyomegaly, hyaline casts, desquamation and parenchyma degeneration of tubular epithelial cells, interstitial nephritis;
↓BW (9%); ≈plasma Na+ and K+
Kidney: ↓GSH, GPx, CAT, ↑MDA | [125] |
| Wistar
(Turkey) | 200-250g
female
N=10 | ip | 7 mg/kg | D5 | ↑8x | ↑4.5x | D5 | 0% | Histology: D5: extensive epithelial cell vacuolization, swelling and desquamation
Kidney: ↓ SOD, CAT, GPx,↑ MDA, MPO | [126] |
| Wistar Hanover
(Harlan) | male
N=5 | iv | 8 mg/kg | D3
D5 | ↑2x
↑5.5x | ↑1.3x
↑6.5x | D5 | 0% | Histology: D5: widespread tubular degeneration and regeneration, tubules contained granular and proteinaceous casts, mineralization (5/5)
D3: ↑AST (1.2x), urine proteins, glycosuria
D5:↓AST, TBIL, GGT, ALP, CI; ↑urine proteins, glycosuria | [64] |
| Wistar Hanover
(Harlan) | male
N=5 | iv | 4x 1 mg/kg | D3
D5 | ns
ns | ns
ns | D5 | 0% | Histology: D5: similar to 8 mg/kg but less extensive (5/5) | [64] |
| | Nephrotoxicity and antitumor activity in rats with cancer |
| | rats | N=3-5 | iv | 5mg/kg | D3
D6 | 51±10 (↑2x)
56±20 (↑2x) / | | D6 | 0% | ↓5% BW | [127] | | |
| | rats | N=3-5 | ip | 3 x 2.5 mg/kg daily | D3
D6 | 34±4 (↑0.5x)
73±28 (↑3x)) / | | D6 | 0% | ↓17% BW | [127] | | |
| | rats | N=3-5 | ip | 7.5 mg/kg | D3
D6 | 60±10(↑0.2x)
216±82 (↑8x) / | | D6 | 0% | ↓22% BW | [127] | | |
|
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