BioMed Research International

Research Article

Comparative Analysis of V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3) Gene between Cow and Buffalo Reveals Substantial Differences for Mastitis

Table 3

List of important consensus sequence motifs and variations observed for AKT3 of cow and buffalo.
MotifsConsensus sequenceRegions Cow sequence RegionsBuffalo sequence
TATA sequenceTATA −1267 to −1271, −1328 to −1332, 
−1365 to −1369, −1863 to −1867, 
−1927 to −1931,		TATA −647 to 651, −656 to −660, −1144 to −1148, −1263 to −1267, 
−1324 to −1328, −1359 to −1363, −1367 to −1371, −1858 to 
−1862, −1906 to −1710, −1922 to −1926,		TATA 
TATAA −649 to −654, −1372 to −1377, 
−1536 to −1541, −2225 to −2230, 
−2289 to −2294, −2657 to −2662, 		TATAA −646 to −651, −1366 to −1371, TATAA 
TATAAA −1145 to −1151, −275 to −280,TATAAA N/A TATAAA  
TATAA 
changed to		−632 to −6637, −658 to −663, 
−677 to −682, 
−733 to −738, −805 to −810, 
−834 to −839, −860 to −864, 
−1053 to −1058, −1076 to −1081, 
−1218 to −1223, −1277 to −1282, 
−1336 to −1341, −1507 to −1512, 
−1813 to −1818, −1904 to −1909,		TGTAA, TGTAA, 
TGTAA, TTTAA, 
TTTAA, TGTAA, 
TGTAA, TTTAA, 
TGTAA, TTTAA, TGTAA, 
TTTAA, TCTAA, TCTAA, 
TTTAA,		−275 to −280, −629 to −634, −674 to −679, 
−730 to 735, 
−802 to 807, −831 to −836, −858 to −863, −1050 to −1055, 
−1214 to 1219, −1273 to −1278, −1332 to 1337, 1501 to − 
1506, −1807 to −1812, 1899 to −1904,		TGTAA, TGTAA, TTTA 
A, TTTAA, TGTAA, TG 
TAA, TTTAA, 
TGTAA, TGTAA, 
TTTAA, TCTAA, 
TCTAA, TTTAA, 
TTTAA,
CRETGACGTCA−219 to −227, −1661 to −1669,TGAGGTCA, 
TGACATGA		−204 to −212, −219 to −227, −1677 to −1685,TGAAGACA, 
TGAGGTCA, 
TGAGGACA,
E-boxCANNTTG−24 to −31, −245 to −252, 
−901 to −908,		CAAGTTG, CAGTTTG, 
CAGATTG,		−24 to −31, −245 to −252, −898 to −905,CAAGTTG, CAGTTTG 
CAGATTG,
ECGTGG−95 to −99, −368 to −372, 
−918 to −922, −950 to −954, 
−988 to −992, −1288 to −1292, 
−1652 to −1656,		GTGG−95 to −99, −367 to −371, 
−915 to −919, −947 to −951, 
−955 to −989, −1284 to −1288, 
−1646 to −1650,		GTGG
CAATCAAT−130 to −134, −398 to −402, 
−525 to −529, −541 to −545, 
−690 to −694, −826 to −830, 
−1271 to −1275, −1379 to −1383, 
−1384 to −1388,		CAAT−130 to −134, −397 to −401, −524 to −528, 
−540 to −544, −687 to −691, −823 to −827, 
−1267 to −1271, −1373 to −1377, −1378 to −1382,		CAAT
NF-KBGGGRNNYY 
CC		−3 to −13, −14 to −24, 
−82 to −92, −847 to −857, 
−1422 to −1432,		GGGAGCCATC, 
GGGGCTCAGC, 
GGGCAGCAGC, 
GGGAATCAGC, 
GGGTTTTTCC,		−3 to −13, −82 to −92, −844 to −854,GGGAGCCATC, 
GGGCAGCAGC,
IRFGAAANNGA 
AAG		 −1036 to −1048, −1539 to −1551GAAACTGATTTT, 
GAAAAAGAACTA		−1033 to −1045, −1533 to −1545,GAAACTGATTTT, 
GAAAAAGAACTG
SP-1 siteGGGCGG−10 to −16, −19 to −25, −85 to −92, 
−916 to −922, −1652 to −1659,		GCGGGG, GGGGGC, 
GGGCAG, GTGGGC, 
GGGGTGG,		−6 to −16, −19 to −25, 
−85 to −92, −913 to −917, 
−1132 to −1136, −1646 to −1653		GAGGGGAGCC, 
GGGGGC, GGGCAGC, 
GGGC, CGGG,GGGGT 
GG,
TSS −95, GGGAAG, 
−1795, ATTTTT		G, T−165, TTGCAA 
−1765, TCTGTC		G, T
CPG islandN/A  N/A 
Predicted promoters −225 to −275, −826 to −876,Identity 100, but showing variation at one position as G/T−225 to −275, −823 to −873,