Transcriptional Profiling of Leucocyte Count Variation from Porcine Peripheral Blood Reveals Differential Gene Expression

Figure 3

Microarray fold change verification with qPCR. (a, b) Fold change of 14 genes of IR and DR, before and after stimulation coded as IRBS/IRAS and DRBS/DRAS, respectively. Data are reported for three technical replicates in all biological samples. Error bars indicate standard error of the mean (SEM). Each histogram represents the level of the target gene relative expression level. Asterisks symbolized P value significance (p ≤ 0.05 (), p ≤ 0.01 (), p ≤ 0.001 (), and p ≤ 0.0001 () based on assuming unequal variances Student’s t-test. (c) qPCR validated 14 genes. A line plot of qPCR log2 (FC) and microarray log2 (FC) data of 14 genes. The linear relationship between the qPCR and microarray log2 (FC) data based on the R² value, and the line linear regression (Y).

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