|
| Lipid extraction | Determination of lipid content | Ref. |
| Reagent(s) | Extraction process | Treatment for final determination | Expression of lipid content |
|
| Chloroform : isopropanol (1 : 1, v/v) andhexane. | Add solvent mixture to frozen pellets; centrifuge and transfer supernatants; reextract pellets with hexane and centrifuge to collect supernatants. | Dry the combined supernatant in a speed vacuum and record the weight. | A percentage of total fresh weight (% w/w) | [21] |
|
| Ethyl ether. | Ground dry samples to powder and use the Soxhlet extractor. | Distill the solvent, dry the residue, and record the weight. | A percentage of dry cell weight (% w/w) | [22] |
|
| Methanol : chloroform : 1% NaCl (2 : 2 : 1, v/v/v). | Extract the biomass with the solvent mixture. | Evaporate the chloroform layer; dry the sample and record the weight. | The weight ratio of extracted lipid to lyophilized pellets (% w/w) | [23] |
|
| Deionized (DI) water : methanol : chloroform (1 : 2 : 1, v/v/v); chloroform. | Add solvent mixture to harvested biomass and react for 24 h; mix and then add solvent to achieve a final DI water : methanol : chloroform ratio of 0.9 : 1 : 1; centrifuge, remove, and filter the lipid-chloroform layer; repeat the above steps for second extraction. | Evaporate the chloroform layer; cool the tube and record the weight. | A percentage of total biomass weight (%, w/w) | [24] |
|
| Chloroform : methanol (2 : 1, v/v). | Mix dry algal powder with the solvent mixture; put under water bath with the aid of ultrasound; centrifuge and repeat the above steps three times. | Evaporate the organic solvent and weight. | A percentage of total biomass weight (%, w/w) | [25] |
|
| Chloroform : methanol (2 : 1, v/v). | Extract lipid from dry biomass with the solvent mixture. | Evaporate the solvent and weight. | Weight difference between the blank flask and the flask containing the extracted oil | [26] |
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