BioMed Research International

Research Article

Comparative Studies of Pectinase Production by Bacillus subtilis strain Btk 27 in Submerged and Solid-State Fermentations

Table 8

Effect of nitrogen sources on pectinase production.


Nitrogen Sources	SmF		SSF
	Enzyme activity	Relative Activity ()	Enzyme activity	Relative Activity
	(U/ml)	Relative Activity ()	(U/g)	()

NH₄Cl	44.7 ± 4.8	73.4	975.7 ± 184.0	84.1
NH₄NO₃	29.4 ± 1.6	48.3	1230.0 ± 30.2	106.0
NH₄SO₄	34.9 ± 2.4	57.3	1261.2 ± 64.0	108.7
Casein	67.7 ± 4.7	111.2	1008.6 ± 19	87.0
Glycine	33.9 ± 0.7	55.7	970.5 ± 43.0	83.7
Peptone	33.8 ± 2.4	55.5	933.4 ± 31.8	80.5
Urea	32.2 ± 3.7	53.0	884.7 ± 83.4	76.3
Yeast Extract	-	-	1076.9 ± 17.2	92.8
Control	60.9 ± 1.1	100.0	1160.0 ± 2.50	100.0

(i) Values are mean ± S.D. of 3 replicates.
(ii) Values followed by different superscripts are significantly different at (P<0.05).
(iii) Values followed by same superscripts are not significantly different at (P<0.05).
(iv) The control is unsupplemented with any nitrogen source.