Research Article

Cloning and Expression of the Organophosphate Pesticide-Degrading α-β Hydrolase Gene in Plasmid pMK-07 to Confer Cross-Resistance to Antibiotics

Table 2

Various genes present in the pMK-07 plasmid.

ORFSequence descriptionGene length (bp)

AP007210.1_9transposase699
AP007210.1_11group II intron reverse transcriptase maturase1233
AP007210.1_14SAM-dependent methyltransferase1092
AP007210.1_18chitin-binding protein1275
AP007210.1_20F0F1 ATP synthase subunit alpha420
AP007210.1_21acid-soluble spore C5216
AP007210.1_22chemotaxis protein819
AP007210.1_23Bacillolysin precursor1671
AP007210.1_28peptidase S8762
AP007210.1_29precorrin-3B C(17)-methyltransferase468
AP007210.1_32conserved hypothetical protein144
AP007210.1_33integrase951
AP007210.1_37nucleotidyltransferase378
AP007210.1_38cytotoxin264
AP007210.1_40DNA-binding protein414
AP007210.1_41nucleotidyltransferase387
AP007210.1_43thiamine biosynthesis849
AP007210.1_50DNA polymerase III subunit beta1113
AP007210.1_56membrane protein285
AP007210.1_59MULTISPECIES: membrane264
AP007210.1_63S1 RNA binding domain984
AP007210.1_64inosine-uridine preferring nucleoside hydrolase family951
AP007210.1_65reverse transcriptase1647
AP007210.1_66conjugation family3501
AP007210.1_67conserved hypothetical plasmid387
AP007210.1_68conserved hypothetical plasmid1287
AP007210.1_69Plasmid replication repX1308
AP007210.1_70conserved hypothetical protein351
AP007210.1_72IS605 family1113
AP007210.1_78IS605 family transposase1335
AP007210.1_79surface layer1278
AP007210.1_81integrase core domain786
AP007210.1_82DNA-binding522
AP007210.1_84Flp pilus assembly861
AP007210.1_85SAF domain family852
AP007210.1_86type II secretion system1425
AP007210.1_87membrane933
AP007210.1_88conserved hypothetical protein867
AP007210.1_90conserved domain204
AP007210.1_91conserved domain207
AP007210.1_92IS605 family transposase1119
AP007210.1_95membrane protein624
AP007210.1_98sortase702
AP007210.1_99LPXTG-motif cell wall anchor domain528
AP007210.1_100ATP-binding protein189
AP007210.1_103conserved hypothetical protein252
AP007210.1_104cell division165
AP007210.1_106recombination U126
AP007210.1_113transposase for insertion sequence element D1275
AP007210.1_115transposon resolvase561
AP007210.1_116S-layer homology domain ribonuclease3396
AP007210.1_117barnase inhibitor276
AP007210.1_119putative membrane protein198
AP007210.1_124MULTISPECIES: ATPase924
AP007210.1_127prgI family351
AP007210.1_130reverse transcriptase1833
AP007210.1_132Reticulocyte binding3981
AP007210.1_133M23 M37 family2208
AP007210.1_139CAAX amino protease705
AP007210.1_141penicillin-binding partial414
AP007210.1_142membrane protein1146
AP007210.1_143thiol reductase thioredoxin483
AP007210.1_147family transcriptional regulator279
AP007210.1_148transcriptional regulator327
AP007210.1_149integrase recombinase1056
AP007210.1_150Transposase (plasmid)165
P007210.1_151conserved domain513
AP007210.1_152transcriptional regulator255
AP007210.1_153conserved domain156
AP007210.1_155type VII secretion1233
AP007210.1_156SMI1 KNR4 family450
AP007210.1_157lumazine binding domain381
AP007210.1_160transcriptional family300
AP007210.1_163transposase, partial630
AP007210.1_164transposon resolvase576
AP007210.1_165family transcriptional regulator846
AP007210.1_166transposon resolvase495
AP007210.1_168N-acetylmuramoyl-L-alanine amidase699
AP007210.1_169phosphoglycerate mutase570
AP007210.1_170IS21 family1254
AP007210.1_171ATPase AAA759
AP007210.1_172Two-component response regulator864
AP007210.1_173glyoxalase family354
AP007210.1_174tn3 transposase DDE domain3054
AP007210.1_175site-specific recombinase627
AP007210.1_177XRE family transcriptional regulator231
AP007210.1_178alpha beta hydrolase732
AP007210.1_180cardiolipin synthetase1494
AP007210.1_181membrane yetF549
AP007210.1_183lipo486
AP007210.1_184stage V sporulation AC477
AP007210.1_185stage V sporulation AD1017
AP007210.1_186stage V sporulation AE351
AP007210.1_187NADH dehydrogenase207
AP007210.1_189ATP-dependent Clp protease proteolytic subunit582
AP007210.1_190resolvase552
AP007210.1_191spore germination C1134
AP007210.1_192spore germination657
AP007210.1_193spore germination family1536
AP007210.1_194phospholipase D competence helix-hairpin-helix domain222
AP007210.1_195transposase1500
AP007210.1_196transposase, partial1695
AP007210.1_197transposase for transposon741
AP007210.1_198transcriptional regulator357
AP007210.1_199S-(hydroxymethyl)glutathione dehydrogenase-like1116
AP007210.1_200S-glutathione hydrolase834
AP007210.1_201UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase273
AP007210.1_202Glutamate--cysteine ligase2268
AP007210.1_204spore germination XA696
AP007210.1_205germination %2C Ger(x)C family1155
AP007210.1_206Spore germination1161
AP007210.1_207cell surface15033
AP007210.1_209Isochorismatase531
AP007210.1_211Two-component response regulator681
AP007210.1_212two-component sensor histidine kinase1848
AP007210.1_214conserved domain1011
AP007210.1_215inosine-uridine preferring nucleoside hydrolase family333
AP007210.1_217cell surface3711
AP007210.1_218cell surface6834
AP007210.1_219RNA-binding Hfq186
AP007210.1_220family transcriptional regulator294
AP007210.1_2212-oxoglutarate dehydrogenase429
AP007210.1_225DNA topoisomerase I2664