Transcriptome Analysis Reveals Dynamic Gene Expression Profiles in Porcine Alveolar Macrophages in Response to the Chinese Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus

Figure 3

qRT-PCR validation of PRRSV infection and differentially expressed genes in PAMs in response to PRRSV at different time points. Shown are the mRNA level of PRRSV N gene (a), the expression patterns of multiple annotated mRNAs (b–e), the expression patterns of two annotated lncRNAs (f, g), and the expression patterns of two novel lncRNAs (h, i). qRT-PCR was performed by using the primers specific for corresponding gene. PPIA served as the reference gene. Error bars represent the standard error of three biological replicates. Asterisks indicate significant differences by Student’s test (; ; ns, not significant).

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