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BioMed Research International
Volume 2018, Article ID 1582173, 11 pages
Research Article

Influence of Adalimumab on the Expression Profile of Genes Associated with the Histaminergic System in the Skin Fibroblasts In Vitro

1Department of Skin Structural Studies, Chair of Cosmetology, School of Pharmacy with Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, ul. Kasztanowa 3, 41-200 Sosnowiec, Poland
2Chair and Department of Molecular Biology, School of Pharmacy with Division of Laboratory Medicine in Sosnowiec, Silesian Medical University, ul. Jedności 8, 41-206 Sosnowiec, Poland
3Department of Internal Medicine and Rheumatology, School of Medicine in Katowice, Medical University of Silesia, ul. Ziołowa 45/47, 40-635 Katowice, Poland

Correspondence should be addressed to Dominika Wcisło-Dziadecka; lp.airetni@akcedaizdd

Received 11 September 2017; Revised 26 November 2017; Accepted 10 December 2017; Published 2 January 2018

Academic Editor: Marija Mostarica-Stojković

Copyright © 2018 Dominika Wcisło-Dziadecka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. The aim of this study was to evaluate the influence of adalimumab on expression profile of genes associated with the histaminergic system in Normal Human Dermal Fibroblast (NHDF) cells stimulated with 8.00 g/ml of adalimumab and the identification of miRNAs regulating these genes’ expression. Methods. NHDFs were cultured with or without the presence of adalimumab for 2, 8, and 24 hours. The expression profile of genes and miRNA were determined with the use of microarray technology. Results. Among 22283 ID mRNA, 65 are associated with the histaminergic system. It can be observed that 15 mRNAs differentiate NHDFs cultures with adalimumab form control. The analysis of miRNAs showed that, among 1105 ID miRNA, 20 miRNAs are differentiating in cells treated with adalimumab for 2 hours, 9 miRNA after 8 hours, and only 3 miRNAs after 24 hours. Conclusion. It was also determined that miRNAs play certain role in the regulation of the expression of genes associated with the histaminergic system. The results of this study confirmed the possibility of using both genes associated with this system as well as miRNAs regulating their expression, as complementary molecular markers of sensitivity to the adalimumab treatment.