Immunological Compatibility of Bone Tissues from Alpha-1,3-galactosyltransferase Knockout Pig for Xenotransplantation

Figure 2

Effects on human PBMC proliferation. PBMCs were obtained from heparinized blood of healthy volunteers by histopaque gradient centrifugation. (a) PBMCs were labeled with CFSE and then bone extracts were added to PBMC cultures at 100 ug/ml concentration. Cells were incubated at 37C° for 72 hrs and analyzed on flow cytometry. (b) Cytotoxicity of bone extracts was evaluated with propidium iodide staining, followed by flow cytometry analysis. #, ##, and ### indicate significant difference from the control group. p < 0.05, p < 0.005, and p < 0.001 indicate significant difference from the WT group. Experiments were repeated at least three times independently with hPBMC from 3-4 blood donors and bone extracts from 2-3 pigs. Data are expressed as means ± SEM.

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