Nucleolar Division in the Promastigote Stage of Leishmania major Parasite: A Nop56 Point of View

Figure 1

Sequence alignment and three-dimensional predicted structure of Nop56 orthologues. (a) Alignment of amino acid sequences of Nop56 from L. major (Lm; LmjF.10.0210), T. brucei (Tb; Tb927.8.3750), T. cruzi (Tc; TCDM_07668), S. cerevisiae (Sc; YLR197W), and H. sapiens (Hs; O00567). Identical residues in all species are indicated by black shading, while conserved residues in four organisms are denoted by gray shading. Trypanosomatid-specific conserved residues are shaded in red. Predicted secondary structure elements in LmNop56 are displayed on top of the linear sequence. β-strands are symbolized by arrows and α-helices by cylinders. The NOP5NT, NOSIC, and Nop conserved domains are indicated in red, green, and blue, respectively. (b) The predicted three-dimensional modeling of Nop56 from L. major (residues 8 to 421), T. brucei (8 to 421), and S. cerevisiae (8 to 417) was obtained with the UCSF Chimera software using the SWISS-MODEL Template Library ID: 5wyj.3.A as a prototype. The three highly conserved domains are colored as indicated in panel (a).

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