Nucleolar Division in the Promastigote Stage of Leishmania major Parasite: A Nop56 Point of View

Figure 3

Expression and subcellular distribution of LmNop56 in L. major promastigotes in stationary growth phase. (a) Western blot analysis of total protein extracts from promastigotes harvested in the mid logarithmic phase (Log), and early (Sta 4) and late (Sta 7) stationary phases. The blots were probed with polyclonal LmNop56 immune serum and with α/β-tubulin antibody (loading control). (b) Indirect immunofluorescence assays performed with cells in Log, Sta 4, and Sta 7 phases using the anti-LmNop56 serum, and an anti-mouse IgG antibody conjugated with Alexa Fluor® 488 dye (green). Nuclear and kinetoplast DNA were counterstained with DAPI (blue). Nucleolar (No) and extra-nucleolar (arrows) green fluorescent signals were visualized with a conventional epifluorescence microscope. Size bars represent 5 μm.

(a)

(b)