Nucleolar Division in the Promastigote Stage of Leishmania major Parasite: A Nop56 Point of View

Figure 4

Intranuclear distribution of LmNop56 during cell division of L. major. Parasites fixed with paraformaldehyde were stained for double indirect immunofluorescence analysis using a mixture of primary antibodies against LmNop56 and α/β-tubulin (to label subpellicular microtubules and mitotic spindle) followed by anti-mouse IgG conjugated with Alexa Fluor® 568 (red) and anti-rabbit IgG coupled with Alexa Fluor® 488 (green) secondary antibodies. DNA was stained with DAPI (blue). (a) Interphase promastigote with (bottom image) and without (top image) brightfield. (b) L. major promastigotes at early, middle, and late stages of closed mitosis were analyzed. DNA, LmNop56, subpellicular microtubules, and mitotic spindle (indicated with a white arrowhead) are visualized. Colocalization of DNA and proteins is displayed in the Merge column. All images were obtained with a conventional epifluorescence microscope. K: kinetoplast; N: nucleus; No: nucleolus. Size bar denotes 2 μm.

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