Nucleolar Division in the Promastigote Stage of Leishmania major Parasite: A Nop56 Point of View

Figure 6

Subcellular localization of Nop56 during mitosis of procyclic T. brucei cells. Double Immunofluorescence assay was conducted in cells fixed with paraformaldehyde and then stained for Nop56 and α/β-tubulin (to detect subpellicular microtubules and mitotic spindle), followed by anti-rabbit IgG coupled with Alexa Fluor® 488 (green) and anti-mouse IgG conjugated with Alexa Fluor® 568 (red) secondary antibodies. Nuclear and kinetoplast DNA were counterstained with DAPI (Blue). (a) Procyclic parasites during interphase. Size bar indicates 5 μm. (b) A set of representative micrographs of parasites in the different steps of closed mitosis is presented. Overlapping of DNA, tubulin, and TbNop56 is shown in the Merge column. Intranuclear mitotic spindle is indicated by a white arrowhead. Images were obtained with a conventional epifluorescence microscope. K: kinetoplast; N: nucleus; No: nucleolus. Size bar denotes 2 μm.

(a)

(b)