A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP

Figure 4

ELISA protein quantification of GF of t-PRP and c-PRP groups. (a) GF quantification of VEGF, PDGF-AB, PDGF-BB, bFGF, EGF, and IGF in plasma, PRP, and activated PRP. Compared to c-PRP, t-PRP showed a relative stabile content of GFs in plasma and PRP, which then decreased greatly in the plasma after activation. (b–d) GFs released from PRP gel quantification at each time point of VEGF ((b), left), PDGF-AB ((c), left), and PDGF-BB ((d), left) over a 96 h period. Total accumulation of GFs over 0.5–96 h for VEGF ((b), right), PDGF-AB ((c), right), and PDGF-BB ((d), right). The release rate of GFs decreased greatly with time in the c-PRP group, while t-PRP showed slower release characteristics. (e–g) GFs degraded in activated PRP quantification at each time point of VEGF (e), PDGF-AB (f), and PDGF-BB (g) over a 72 h period. VEGF was not significantly different between groups, whereas PDGF-AB and BB degraded slowly in t-PRP. ; .

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