Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis

Figure 1

Distribution and expression of LIC13479 and LIC10050 genes in Leptospira spp. (a) Genomic DNA from L. interrogans serovars: Copenhageni (1), Canicola (2), Icterohaemorrhagiae (3), Pomona (4), Hardjo (5), L. borgpetersenii serovar Whitticombi (6), L. kirshneri serovars Cynopteri (7) and Grippotyphosa (8), L. santarosai serovar Shermani (9), L. noguchii serovar Panama (10), and saprophytic species L. biflexa serovar Patoc (11) were subjected to PCR analysis with specific primers. The integrity of the DNA used was determined by amplification of 16S DNA. (b) Transcript levels of the LIC13479 and LIC10050 were determined by qRT-PCR in virulent L. interrogans serovar Copenhageni strain FIOCRUZ L1-130 and culture-attenuated L. interrogans strain M20. The relative gene expression was calculated by method after normalization with 16S. Error bars show the mean ± SD from three independent experiments performed in triplicate.

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