Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis

Figure 2

Immune response induced by immunization with Lsa46. Hamsters were inoculated subcutaneously with Lsa46 combined with 12.5% Alum and booster was administered after two weeks. Immunized animals with PBS or bacterin were used as negative and positive control, respectively. Animals were bled two weeks after each immunization and sera were used to determine antigen-specific total IgG by ELISA (a) and IgG1 and IgG2/3 (b). The IgG1/IgG2/3 ratios are shown in (c). Error bars show the mean ± SD from two independent experiments performed in triplicate. Representative data are shown.

(a)

(b)

(c)