Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis

Figure 3

Antibodies induced by immunization with Lsa77. Hamsters were immunized subcutaneously with Lsa77 combined with 12.5% Alum and booster was administered after two weeks. Inoculated animals with PBS or bacterin were used as negative and positive control, respectively. Animals were bled two weeks after each immunization and sera were utilized to determine antigen-specific total IgG (a) and IgG1 and IgG2/3 (b) response by ELISA. The IgG1/IgG2/3 ratios are shown in (c). Error bars show the mean ± SD from two independent experiments performed in triplicate. Representative data are shown.

(a)

(b)

(c)