Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis

Figure 4

Humoral immune response induced by immunization with the combined recombinant proteins. Hamsters were immunized subcutaneously with Lsa46 and Lsa77 combined with 12.5% Alum; booster was administered after two weeks. PBS or bacterin immunized animals were used as negative and positive control, respectively. Animals were bled two weeks after each immunization and sera were utilized to determine Lsa46-specific total IgG, IgG1 and IgG2/3 response ((a) and (c)) or Lsa77-specific response ((b) and (d)). The IgG1/IgG2/3 ratios are shown in (e). Error bars show the mean ± SD from two independent experiments performed in triplicate. Representative data are shown.

(a)

(b)

(c)

(d)

(e)