Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis

Figure 5

Protective effect induced by immunization with Lsa46 and Lsa77. Animals were immunized subcutaneously with Lsa46 (a), Lsa77 (b), or combined Lsa46 and Lsa77 (c) with 12.5% Alum; booster was administered after two weeks. Inoculated animals with PBS or bacterin were used as negative and positive control, respectively. Two weeks after the second immunization, animals were challenged intraperitoneally with 1x10⁴ L. interrogans serovar Kennewicki strain Pomona Fromm. After challenge, hamsters were monitored for 21 days. Two independent experiments were performed and pictures represent the data of experiments 1 and 2. Rates of survival were compared using log-rank and Fisher’s exact test. P-value < 0,05 was considered significant.

(a)

(b)

(c)