Research Article
Identification and Validation of Reference Genes for RT-qPCR Normalization in Mythimna separata (Lepidoptera: Noctuidae)
Figure 3
Optimal number of reference genes for normalization in M. separata. (a) Pairwise variation ( / ) was analyzed between the normalization factors and to determine the optimal number of reference genes required for accurate normalization. A value < 0.15 denotes that additional reference genes will not markedly improve normalization. (b) Correlation between the NF of most three stable and optimal number endogenous control. Pearson’s correlations between the NFs of three endogenous control genes (NF3) and optimal number (y-axis) of endogenous control genes () for (A) developmental stages, (B) temperature treatments, (C) biotic factors, and (D) all samples.
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